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KMID : 0616120190500030226
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Korean Journal of Pharmacognosy
2019 Volume.50 No. 3 p.226 ~ p.231
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Isolation and Quantitative Analysis of Naringenin, Sakuranetin and Prunetin from the Barks of Prunus yedoensis
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Kim Eun-Nam
Kim Sung-Cheol
Jeong Gil-Saeng
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Abstract
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Prunus yedoensis Matsum. (Rosaceae) has been used for cough, urticaria, pruritus, dermatitis, asthma and relaxation in traditional Korean medicine. In this study, naringenin, sakuranetin and prunetin were isolated from the barks of P. yedoensis and quantification were performed by using high performance liquid chromatography (HPLC) method with diode array detector. The structures of naringenin and sakuranetin, prunetin were identified NMR spectroscopic data analysis. The content of each compound was analyzed by HPLC and the analytical method was validated through linearity, precision, accuracy and specificity test. The result showed that calibration curves of three compounds naringenin, sakuranetin and prunetin indicated great linearity with a correlation coefficient (R²) of 1.00, 1.00 and 0.99. Intra and inter day measurement accuracy of the three compounds ranged from 92.70 ~ 112.70%, and showed precision was less than 3%. Therefore, the content analysis showed that naringenin (0.132 ± 0.016%), sakuranetin (0.108 ± 0.023%), and prunetin (0.059 ± 0.014%).
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KEYWORD
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HPLC, Prunus yedoensis Matsum., Sakuranetin, Naringenin, Prunetin
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